Platelet/Thrombocyte count

 

Apparatus and reagents

  • Dry clean glass slide
  • reagent (MgSO4)
  • Reese-Ecker fluid
  • disposable lancet.

Principle

Platelet/thrombocyte count is very high, the blood is therefore diluted 200 times, with appropriate diluting fluid and then the stained cells are counted in a hemocytometer.  Their number in undiluted blood can then be calculated.

Direct method

In this method, diluting fluid used in Reese-Ecker fluid. Its different components have the following characteristics:
  • Brilliant cresyl blue          =>               Stains the platelets
  • Formalin                        =>               prevents fungal growth
  • Sodium citrate                 =>               makes the fluid isotonic with blood

Identification of platelets

  • Small, membrane bounds, non-nucleated granular bodies.
  • Oval is disc-shaped with condensed cytoplasm in the center, giving the impression of the nucleus.
  • Possess an extreme surface insatiability and throughout long pseudopodia.
  • Rapidly stick to and spread around other platelets or objects like glass.

Procedure

  1. Filter the Reese-Ecker fluid through double filter paper and draw it in the RBC diluting pipette up to the mark 0.5.
  2. Wipe the tip and then draw blood from a finger prick to the 0.5 marks so that the platelet solution reaches the mark 1.
  3. Wipe the tip again and fill the pipette with platelet solution to the mark 101.
  4. Mix the contents for 2 minutes.
  5. Discard the 1st 3-4 drops and charge the counting chamber with the diluted blood.
  6. Count the platelet in five groups of 16 squares each (as for RBC, see at).
  7. Calculate their number in undiluted blood by following the same formula as for RBC counting (see at).

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