Cultivation of molds on the solid surface

Cultivation of molds on the solid is sometimes preferred when fungal colony morphology examination is required. Sometimes fungi can be identified by observing their colony shape, appearance, and color. Such as rotting citrus fruit have blue-green velvety growth that is penicillium. Cheese mostly carry mucor species that appear grayish white furry. While black stalk appearance belongs to Rhizopus.

Materials

  • Fungal growth sample
  • Sabouraud agar plates
  • Potato dextrose agar plates
  • Bunsen burner
  • Sterile normal saline
  • Inoculation loop

Procedure

  1. Label Petri plates according to the sample name (one of Sabouraud and one of potato dextrose agar for each sample).
  2. Add some normal saline in a separate test tube for each sample.
  3. With the inoculation loop scratch, a few loopful cultures from the sample source and add in normal saline and mix well by gentle tapping.
  4. Inoculate each of the agar plates from the normal saline suspension. Never perform spreading just inoculate in the center of the Petri plate.
  5. Incubate all the plates at 25°C for 7-10 days.
  6. Observe colony morphology and appearance.

Comments

Post a Comment

Popular posts from this blog

Sugar •        Sugar has been described both in history and recently as a topical wound treatment. •        The basis for its use is its high osmolality. •        Thus, it is effective in reducing edema due to its hydrophilic action. •        It has also been reported to attract macrophages, accelerate slough of devitalized tissue, provide cellular energy, and promote granulation tissue. •        Its use should be discontinued after the wound has a good bed of granulation tissue. •        It has been reported to be effective against Staph., Strep., Enterobacter, E. coli, Klebsiella, Pseudomonas, and Serratia organisms. •          It reportedly does not cause hyperglycemia. •        It should be used with caution on large wounds. Its hydrophilic action can pull more fluids, electrolytes, and proteins from the wound tissues.  
Read more

Nematodes

Image
    General Characteristics: Nematodes are commonly called roundworms because, as the name suggests, they are round when viewed in cross section. They are cylindrical in structure and taper towards their anterior and posterior ends. They are bilaterally symmetrical. The sexes are separate in most species, a few are hermaphrodite. Nematodes that parasitize domestic animals are found in all parts of the body but are most commonly found in the digestive and respiratory tracts, and in the circulatory system.
Read more
PHYSICAL EXAM PROCESS 1)  Signalment / History 2)  General Appearance / Initial Observations 3)  Vital Signs 4)  Physical Exam (Systems Approach or Head to Toe) 5)  Surgical / Anesthetic Risk Assessment 6)  RAVS Animal Condition (RAC) Score
Read more