Knowing of bacteriophage in laboratory     

For the growth of bacteriophage, we can use either a liquid or solid medium.

Procedure 

  1. Mix melted agar with bacteriophage (T4) suspension and host bacteria (E-coli).
  2. Take Petri plate and pour MacConkey agar on it.
  3. Pour mixture on agar.
  4. Incubate the plate, after a certain incubation period, there is the formation of the clear zone on the plate.
  5. Cut agar with a sharp blade and place it in chloroform in a test tube. It will separate cell debris and the media part from bacteriophage.
  6. Collect virus by filtration.
   

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