Collection of samples for laboratory analysis


 

Toxin screening

Select tests based on suspected toxin

Sample: Collection and storage of specimen

Sampling includes


  • Suitable material
  • A sampling at the correct point of time
  • Sufficient quantity
  • Suitable sampling techniques
  • Adequate container (should be inert)
  • There should be unique labeling
  • Appropriate storage, transportation, disposal, and handling

There are three types of sampling for toxicological analysis

  1. Antemortem
  2. Post mortem
  3. Environmental sample

Sr. no.
Sample
Amount
Additional information
Antemortem
1
Whole blood
5-10 ml
EDTA, Heparin
2
Serum
5-10 ml
Remove clotting factor
3
Urine
25-50 ml
Plastic screw capped tube
4
GIT content
100 g

5
Milk
30 ml

6
CSF
1-2 ml

7
Hair
1-2 g

Post mortem
8
Whole blood (mainly from heart)
As per antemortem
9
Serum, urine
As per antemortem
10
Liver
100-250 g

11
Kidney
100-250 g

12
Brain
½ of brain

13
GIT content
100 g

14
Spleen
100 g

15
Lung
100 g

Environmental sample
16
Bait/source (fodder)
200 g or ml
In clean plastic jar
17
Feed
200-500 g

18
Plant
Entire plant or the specific part including roots
It should be fresh, or pressed and dry
19
Water
1 liter
In clean jar



Guideline for post-mortem


  1. Take the first sample for microbiological analysis
  2. Take sufficient amount of sample
  3. There should be no cross contamination
  4. Take 2 sets of sample for each tissue
  5. For histopathology, preserve sample in 10 % buffer formalin  (Ratio: Buffer: tissue:: 10 : 1)

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